2-Oxo-1,3-dioxoles as Specific Substrates for Measurement of Arylesterase Activity.

Arylesterase Phenotype-Specific Positive Association Between Arylesterase Activity and Cholinesterase Specific Activity in Human Serum

CONTEXT Cholinesterase (ChE) specific activity is the ratio of ChE activity to ChE mass and, as a biomarker of exposure to cholinesterase inhibitors, has a potential advantage over simple ChE activity. OBJECTIVE To examine the association of several potential correlates (serum arylesterase/paraoxonase activity, serum albumin, sex, age, month of blood collection, and smoking) with plasma ChE s...

Basic Task-specific Ionic Liquid as Catalyst and Reaction Medium: A Green Protocol for the Synthesis of 4-Oxo-6-aryl-2-thioxo-1,2,3,4- tetrahydropyrimidine-5-carbonitrile Derivatives

Abstract A novel basic ionic liquid based on imidazolium cation is designed, synthesized and successfully used as a catalyst for the one-pot synthesis of 4-Oxo-6-aryl-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carbonitrile derivatives. The remarkable features of this new catalyst are its ethyleneoxy bridge which participates in dissolving organic compound in the ionic liquid and its strong base co...

Mining bacterial genomes for novel arylesterase activity

One hundred and seventy-one genes encoding potential esterases from 11 bacterial genomes were cloned and overexpressed in Escherichia coli; 74 of the clones produced soluble proteins. All 74 soluble proteins were purified and screened for esterase activity; 36 proteins showed carboxyl esterase activity on short-chain esters, 17 demonstrated arylesterase activity, while 38 proteins did not exhib...

PARAOXONASE AND ARYLESTERASE ACTIVITY AMONG HYPERCHOLESTEROLEMIC PATIENTS

Human serum paraoxonase (PON) associated with high density lipoprotein (HDL) , has been postulated to have a role in protecting low-density lipoprotein (LDL) against oxidative damage, which has led to the proposal that PON is an anti-atherogenic and anti-inflammatory enzyme. It has genetic polymorphism at the 191 (R ->Q) and two alloenzymes A and B and three phenotypes A, B, and AB. We exa...

Cover for Volume.13, No.2